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Bacterial Metabolism by H. W. Doelle

By H. W. Doelle

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It is relatively easy to determine the constant Km experimentally. In Fig. , when all the enzyme is converted to the complex ES. The mathematical expression for the enzyme velocity is v = V \Km + S/ where v is the reaction velocity, S is the substrate concentration, Km is the Michaelis-Menten constant, and V is the maximal velocity of the reaction. If the variables v and S are then taken as reciprocals and drawn graphically, V and Km can easily be determined on their corresponding coordinates (Fig.

The group includes substances with many different functions, but they all appear to act through oxidation and reduction of the iron. The nomenclature of individual cytochromes has become difficult owing to the isolation from different sources of cytochromes with similar properties. The recommendations of the IUB in regard to the classification and nomencla­ ture can be found in Chapter 5 of the Commission's report (40). Four major groups of cytochromes have been established as follows: 1. , heme a.

J. 13, 167. , eds. (1972). " Academic Press, New York. Questions 1. 2. 3. 4. 5. 6. 7. What is energy? What is the first law of thermodynamics? What is enthalpy? What is the second law of thermodynamics? What is entropy? What is the third law of thermodynamics? " QUESTIONS 37 8. What is the importance of the equilibrium constant K in chemical reactions? 9. Explain the formula Δ ^ = Δ^° X RT In K. 10. O. 038 M. Calculate the standard free energy change of this reaction. 11. How do you calculate the overall feasibility of reactions involved in a metabolic pathway?

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