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Biological Reactive Intermediates—II: Chemical Mechanisms by Robert Snyder

By Robert Snyder

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R. J. , Academic Press, London. , Ioannides, C. and Parke, D. , 1980b, Ligand binding of safrole to cytochrome P-450, Arch. 4:45. , Ioannides, C. -Biol. Interactions, 32:101. , 1977, Inhibition of mixed-function oxidations by substrates forming reduced cytochrome P-450 metabolite-intermediate complexes, Pharmac. , 2:227. , Kim, Y. , 38:3385. , 1979, Isolation and purification of cytochrome P-450, and the existence of multiple forms, Pharmac. , 6:99. C. , 1980, Unpublished data. , 1979, Comparative aspects of the distribution of cytochrome P-450 dependent mono-oxygenase system: an overview.

1977, Inhibition of mixed-function oxidations by substrates forming reduced cytochrome P-450 metabolite-intermediate complexes, Pharmac. , 2:227. , Kim, Y. , 38:3385. , 1979, Isolation and purification of cytochrome P-450, and the existence of multiple forms, Pharmac. , 6:99. C. , 1980, Unpublished data. , 1979, Comparative aspects of the distribution of cytochrome P-450 dependent mono-oxygenase system: an overview. , 10:15. , Richards, R. , 1977, Drug metabolism in the ferret: Effects of age, sex and strain.

Phenobarbitone 32 0 L() a: - 24 ~ 16 >- u 0 E c ...... Ill _. c Control I 8 0 _. _....... _ ·-·-·-• 3-Methylcholanthrene 0 _. e-----•--------so 40 10 20 Phenobarbitone 30 Concentration of carcinogen (pg I plate) Fig. 4. Efficiency of Cylochromes P-450 and P-448 in Generating Mutagens from 2-Acelamidofluorene and 3,4-Benzo(a)pyrene. Experiments were performed on Salmonella strain T A 98. The compounds dissolv:& in DMSO were added lo molten lop agar containing l-3 x 10 bacteria and traces of histidine and biotin.

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